茶黄素(TF3)对射频辐射小鼠肝脏脂肪代谢能力的影响研究开题报告

1. 研究目的与意义

研究背景

茶黄素（tf3）由roberts发现，最初单指红茶中溶于有机相中呈橙黄色的物质，该类物质由多酚类及其衍生物在酶等物质的作用下氧化缩合而来。红茶中茶黄素类的含量一般占红茶干重的2%-6%。茶黄素具有多种与人体健康有关的潜在功能，如抗氧化、防治心血管疾病、降血脂、抗癌防癌等。

2. 研究内容和预期目标

研究内容

在本实验室前期对茶黄素的研究基础上，以c57bl小鼠为研究对象，模拟昼夜环境室温饲养，以不同剂量茶黄素灌胃处理两周，灌胃后辐射一周。通过测量肝脏脂肪代谢指标，探究茶黄素对射频辐射小鼠肝脏脂肪代谢功能损伤的防护效应。

3. 研究的方法与步骤

研究方法

spf级别野生型（wild type，wt）c57bl/6雄性小鼠共50只适应性饲养一周后，将小鼠随机分为空白组、溶剂对照组、辐射（rf）组、rf 12.5 mg/kg tf3组、rf 25 mg/kg tf3组、rf 50 mg/kg tf3组、rf 100 mg/kg tf3组；小鼠于辐射前灌胃14天，进行辐射7天，每天辐射2 h。照射频率为2.1 ghz。

4. 参考文献

[1] Jaman F, Tiwari R C. Environmental and biological effects of cell phone radiation: a review[J]. Design Engineering, 2021, 9: 300-311.
[2] Vamsy A, Lakshmi V S, Venkatachalam T S P. A Study on the Histological and Biochemical Effects of Long-Term Exposure of 4G LTE Radiation Emitted by Mobile Phone on The Liver of Wistar Rats[J]. Asian Journal of Pharmaceutical Research and Health Care, 2021, 13(2): 132-138..
[3] Hasan I, Islam M R. Biochemical and histopathological effects of mobile phone radiation on the liver of Swiss albino mice[J]. European Journal of Anatomy, 2020, 24(4): 257-261.
[4] Pardhiya S, Gaharwar U S, Gautam R, et al. Cumulative effects of manganese nanoparticle and radiofrequency radiation in male Wistar rats[J]. Drug and Chemical Toxicology, 2022, 45(3): 1395-1407.
[5] Mugunthan N, Anbalgan J, Meenachi S. Structural changes observed in mice liver due to chronic Exposure of 1900-2200MHZRadiations Emitted from mobile phone[J]. International Journal of Anatomy and Research, 2019, 7(1.3).
[6] Pooladi M, Montzeri A, Nazarian N, et al. Effect of WiFi waves (2.45 GHz) on aminotransaminases (ALP, ALT and AST) in liver of rat[J]. Archives of Advances in Biosciences, 2018, 9(2): 13-20.
[7] Mugunthan N, Samy K S, Anbalagan J, et al. Long term exposure of 900-1800 MHz mobile phone radiation on mice liver-A histological study[J]. National Journal of Clinical Anatomy, 2016, 5(4): 196-201.
[8] Ragy M M. Effect of exposure and withdrawal of 900-MHz-electromagnetic waves on brain, kidney and liver oxidative stress and some biochemical parameters in male rats[J]. Electromagnetic biology and medicine, 2015, 34(4): 279-284.
[9] Holovska K, Alm#353;iov V, Cigankova V, et al. Structural and ultrastructural study of rat liver influenced by electromagnetic radiation[J]. Journal of Toxicology and Environmental Health, Part A, 2015, 78(6): 353-356.
[10] Usikalu M R, Rotimi S O, Achuka J A. Effects of 900 MHz radiofrequency radiation on the rats’liver[J]. Jurnal Teknologi, 2016, 78(6): 19-24.
[11] Sundaram V, Mohammed S, Cockburn B N, et al. Effects of Intermediate Frequency (150 kHz) Electromagnetic Radiation on the Vital Organs of Female Sprague Dawley Rats[J]. Biology, 2023, 12(2): 310.

5. 计划与进度安排

2024-02-28～2024-03-12 查阅资料，撰写开题报告

2024-03-13～2024-04-01 收集相关文献进行预实验